Abstract:
The mechanisms of action of Cu2+ in improving the longevity of cut flowers and foliage have not been elu cidated. Possible antimicrobial action of Cu2+ against stem end and vase solution colonising bacteria was
investigated using Cu2+ treatments optimised for cut Acacia holosericea A. Cunn. ex G. Don foliage stems.
These treatments were a 5 h pulse with 2.2 mM Cu2+ or a 0.5 mM Cu2+ vase solution versus a deionised
water (no Cu2+) control. Bacterial growth over time was assessed by a standard plate count agar technique
and with scanning electron microscopy. Cu2+ treatments significantly extended the cut foliage vase life.
However, they did not have sustained antibacterial activity against stem end or vase solution colonising
bacteria. Also, regular recutting of 1–2 cm from the stem ends did not substantially improve either cut
stem water relations or longevity. The positive effects of Cu2+ treatments were unaffected by the repeated
stem end recutting. It was concluded that the primary mechanism of Cu2+ was not antibacterial. More over, naturally growing vase solution and stem end microbial populations had relatively insignificant
effects on cut A. holosericea vase life. Research into alternative mechanisms of Cu2+ is required.
